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pbluescript ii ks vector  (New England Biolabs)


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    New England Biolabs pbluescript ii ks vector
    Pbluescript Ii Ks Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript ii ks vector/product/New England Biolabs
    Average 99 stars, based on 3543 article reviews
    pbluescript ii ks vector - by Bioz Stars, 2026-05
    99/100 stars

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    Image Search Results


    cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into pBluescript KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA

    Journal: Cell & Bioscience

    Article Title: A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells

    doi: 10.1186/s13578-021-00618-0

    Figure Lengend Snippet: cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into pBluescript KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA

    Article Snippet: The purified cyp1a1 PCR product from each mutant cell line and the cloning vector pBluescript KS + were digested with SmaI (New England Biolabs, Ipswich, MA, USA).

    Techniques: Sequencing, Clone Assay, Mutagenesis